"Inactivation of Animal and Human Prions by Hydrogen Peroxide Gas Plasma
C, Yousfi R, Soufflet C, Quadrio I, Yan ZX, Huyot V, Aubenque C, Destrez P, Roth K, Roberts C, Favero M, Clayette P.
Infect. Control Hosp. Epidemiol.
Volume 30 (8)
PMID: 19563265 [PuMed - indexed
A quote from the following
"Gaseous or vaporized hydrogen peroxide efficiently inactivates prions on the surfaces of medical
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Prions cause various transmissible spongiform
encephalopathies. They are highly resistant to the chemical and physical decontamination and sterilization procedures routinely
used in healthcare facilities. The decontamination procedures recommended for the inactivation of prions are often incompatible
with the materials used in medical devices. In this study, we evaluated the use of low-temperature hydrogen peroxide gas plasma
sterilization systems and other instrument-processing procedures for inactivating human and animal prions. We provide new
data concerning the efficacy of hydrogen peroxide against prions from in vitro or in vivo tests, focusing on the following:
the efficiency of hydrogen peroxide sterilization and possible interactions with enzymatic or alkaline detergents, differences
in the efficiency of this treatment against different prion strains, and the influence of contaminating lipids. We found that
gaseous hydrogen peroxide decreased the infectivity of prions and/or the level of the protease-resistant form of the prion
protein on different surface materials. However, the efficiency of this treatment depended strongly on the concentration of
hydrogen peroxide and the delivery system used in medical devices, because these effects were more pronounced for the new
generation of Sterrad technology. The Sterrad NX sterilizer is 100% efficient (0% transmission and no protease-resistant form
of the prion protein signal detected on the surface of the material for the mouse-adapted bovine spongiform encephalopathy
6PB1 strain and a variant Creutzfeldt-Jakob disease strain). Thus, gaseous or vaporized hydrogen peroxide efficiently inactivates
prions on the surfaces of medical devices.